尿液外泌体和自由循环RNA提取试剂盒（小提）-Urine Exosome and Free-Circulating RNA Isolation Mini Kit-细胞分析试剂盒-艾美捷科技有限公司

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细胞分析试剂盒

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产品描述: This kit provides a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes ranging from 250 µL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA.The kit provides a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

产品特点: Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile sample input ranging from 250 µL to 1 mL; Isolate all sizes of free-circulating RNA, including microRNA; Bind and elute all RNA irrespective of size or GC content, without bias; The purified exosomal RNA is free from any circulating RNA-binding proteins; No phenol extractions, Proteinase K treatment nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes are required; No precipitation reagents nor overnight incubation required; Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL; Purify high-quality RNA in 15-20 minutes.

其他: Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。