Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or green fluorescent Cell Stress and Calcium sensors can easily be co-expressed with this assay.
产品描述
测量环AMP（cAMP）信号传导动力学，筛选Gs活性的GPCR，以及使用活细胞、荧光、cADDis cAMP测定的更多功能。将cADDis传感器添加到您的细胞中，并在第二天在自动酶标仪或荧光成像系统上测量稳健的信号。增加的红色荧光指示cAMP增加。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括cAMP传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（B2 AR BacMam和10 mM异丙肾上腺素HCl溶液）。10 mL和30 mL的尺寸也可用。其他Montana分子工具，包括BacMam包装的GPCR或绿色荧光细胞应激和钙传感器，可以很容易地与该测定共表达。
