使用高N.A.荧光显微镜,用绿色cADDis监测初级纤毛中cAMP的变化。使用5HT6受体进行纤毛定位。表示用于比率测量的红色荧光标签。还提供了仅针对纤毛的绿色cAMP传感器。CMV启动子。
艾美捷 Montana Molecular定量Cilia靶向cADDis cAMP测定试剂盒#D0211G包含的材料:
比率计量的靶向纤毛cADDis cAMP传感器BacMam
丁酸钠,500 mM水溶液
用红色荧光标签靶向纤毛的绿色荧光cAMP传感器用于比率测量。
Montana Molecular-cAMP部分出版参考:
X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)
L. Bridge, et al. Computational modelling of dynamic cAMP responses to GPCR agonists for exploration of GLP-1R ligand effects in pancreatic β-cells and neurons. Cellular Signaling, April 2024.
R. Fagan, et al. Selective targeting of mu opioid receptors to primary cilia. Cell Reports. April 2024.
G. Austin, et al. An inter-organelle contact between endosomal GLP-1R, ER VAP-B, and the mitochondrial AKAP SPHKAP triggers PKA-dependent MIC19 phosphorylation and β-cell mitochondrial remodelling. bioRxiv. April 2024.
I. Cattani-Cavalieri, et al. Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ. Journal of Visualized Experiments. March 2024.
B. Polacco, et al. Profiling the proximal proteome of the activated μ-opioid receptor. nature chemical biology. March 2024.
P. Balraj, et al. Kisspeptin/KISS1R Signaling Modulates Human Airway Smooth Muscle Cell Migration. American Journal of Respiratory Cell and Molecular Biology. March 2024.
相关产品:
#D0241G Green Membrane-Targeted fS15 cADDis cAMP
#U0600G Green GECO Ca2+ Assay Kit
#U0600R Red GECO Ca2+ Assay Kit
#U0200G Green Up cADDis cAMP Assay Kit
Montana Molecular产品主要包括GPCR检测、细胞应激和毒性相关检测工具,为客户提供了长时间内观察候选药物对细胞过程影响的能力。艾美捷科技是Montana Molecular的中国代理商,为科研工作者提供优质的产品与服务。
微信扫码在线客服