- 中文名称
TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued
- 英文名字
- TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued
- 供应商
- USBiological
- 产品货号
- T9162-80
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Suitable for the detection of the endonucleolytic cleavage of chromatin characteristic of apoptosis.Most animal cells can self-destruct via an intrinsic program of cell death that may be triggered by extracellular or intracellular signals. Programmed cell death plays an important role in many biological processes such as morphogenesis and negative selection in the immune system. In addition, dysregulation of programmed cell death may contribute to diseases, for example, cancer, neurodegenerative diseases, and AIDS. Apoptosis is the term for programmed cell death characterized by specific morphologic and biochemical properties. Apoptotic cells typically undergo a series of structural changes: blebbing of the plasma membrane; condensation of the cytoplasm and nuclei; and cellular fragmentation into apoptotic bodies, membrane-bound structures containing condensed cytoplasm, some intact organelles, and nuclear fragments. The most common biochemical property of apoptosis is the endonucleolytic cleavage of chromatin, initially to large fragments of 50-300 kilobase pairs and subsequently to monomers and multimers of 180-200 base pairs. The TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) method identifies apoptotic cells in situ by using terminal deoxynucleotidyl transferase (TdT) to transfer biotin-dUTP to the free 3'-OH of cleaved DNA. The biotin-labeled cleavage sites are then visualized by reaction with fluorescein conjugated avidin (avidin-FITC).Quality Control Testing:Detected cleaved DNA in HeLa cells after incubation with bovine DNase I Capacity: 40 assays using 50ul end-labeling reaction. Kit Components: T9162-80A: Proteinase K (PK), 1x400ugT9162-80B: TdT, (Terminal deoxynucleotidyl Transferase), 1x300u T9162-80C: TdT Buffer, 3x2mlT9162-80D: dUTP (Biotin), 1x100ul*T9162-80E: TB Buffer (10X), 1x50ml*T9162-80F: Blocking Solution, 1x2mlT9162-80G: Avidin (FITC), 1x10ugT9162-80H: Bovine DNase I, 1x200ugStorage and Stability:Store *T9162-80E and T9162-80F at 4°C once thawed. Store other components at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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