The QuickZyme Sensitive Tissue Hydroxyproline assay is used for the quantitative determination of hydroxyproline. The QuickZyme Sensitive Tissue Hydroxyproline assay kit is the only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate enabling measurement in small samples with low hydroxyproline concentrations. In addition no drying of hydrolydsate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay.