This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts). The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
产品特点
Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction; Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required; Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits; High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products; Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility.