TrueBlot® Protein A Magnetic Beads IP/Co-IP Kit - KBA-PA00-18-TrueBlot® Protein A Magnetic Beads IP/Co-IP Kit

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背景资料: TrueBlot® Protein A Magnetic Beads IP/Co-IP Kit allows highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of an antigen using smaller amounts of antibody (typically 1-10 µg). The kit is designed for immunoprecipitation of proteins, protein complexes, protein-nucleic acid complexes and other antigens. The specific antibody is added to the sample to form an immune complex. The immune complex is then bound to Protein A Magnetic beads. The complex is washed to remove unbound material and the bound immune complex is then eluted from the beads using low-pH elution buffer. Alternatively, the sample buffer is used for dissociation using denaturing conditions or for down-stream process for SDS-PAGE analysis. The kit includes Protein A magnetic beads for high capture efficiencies and optimized buffers for high yield of target antigen. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL. The kit contains magnetic beads, Binding/Wash buffer, Elution buffer, and Neutralization buffer sufficient for 20 samples or tests. (See protocol for details). The beads are removed from the solution using a magnetic separator.

产品描述: TrueBlot® Protein A magnetic beads IP/Co-IP kit can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein A magnetic beads are then incubated with antigen-antibody complex at room temperature, washed and then eluted using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. For antibody purification, Protein A magnetic beads are incubated with the antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually.

产品特点: 该TrueBlot® Protein A Magnetic Beads IP/Co-IP Kit - KBA-PA00-18的详细信息查看Rockland提供的产品说明书。

保存建议: The Protein A Magnetic Beads should be stored in the refrigerator (2-8 °C). The reagent must be allowed to reach room temperature (20-25 °C) before use and may be used until the expiration date. Do not freeze, dry, or centrifuge the beads as they may result in loss of binding activity and aggregation. See kit insert for storage components.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。