人ProIGF-II E(78 - 88)抗血清通过用衍生自连接到白喉类毒素的人IGF-II E的E结构域的11个氨基酸的肽免疫兔来制备。 该序列对应于IGF-II E77 - 88,因此,抗血清识别全长IGF-II E和两种IGF-II E变体(IGF-II E1 - 88和IGF-II E1 - 104),这两种变体来源于人igf-2基因的加工和随后的蛋白水解切割。 它不识别成熟的IGF-II或IGF-I。 序列分析表明,它可能识别非人灵长类动物、狗、豚鼠、猪和许多其他物种中的IGF-II E蛋白。 Human ProIGF-II E (78-88) antiserum was prepared by immunising rabbits with an 11 amino acid peptide derived from the E domain of human IGF-II E linked to diphtheria toxoid. The sequence corresponds to IGF-II E77-88and as a consequence, the antiserum recognises full length IGF-II E and both IGF-II E variants (IGF-II E1-88 andIGF-II E1-104)derived from processing of the human igf-2 gene and following proteolytic cleavage. It does not recognise mature IGF-II or IGF-I. Sequence analysis suggests it is likely to recognise IGF-II E proteins in non-human primates, dog, guinea-pig, pig and a number of other species.
GroPep Bioreagents公司总部位于澳大利亚,成立于1990年,致力于向广大科研院所、生物技术及制药公司开发、生产和销售细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂,现有产品超过100多个,同时在世界范围内均设有分销商。公司在分子生物领域、发酵学及重组蛋白和单抗制备方面具有丰富的经验,在制造工艺方面,拥有大量专利技术,GroPep目前已通过AS/NZS ISO 9001:2016质量管理体系认证。