人ProIGF-I EA(92 - 105)抗血清是通过用衍生自与白喉类毒素连接的人ProIGF-I EA的E结构域的15个氨基酸的肽免疫兔来制备的。 该肽位于153个氨基酸的ProIGF-I EA的C末端,该ProIGF-I EA来源于人igf-1基因的可变剪接。 抗血清识别ProIGF-I EA,但不识别ProIGF-I EB、ProIGF-I EC、成熟IGF-I或IGF-II。 序列分析表明,由于igf-1基因的选择性剪接,它可能在包括非人灵长类、兔、豚鼠、狗、马、绵羊和山羊在内的许多其它物种中识别ProIGF-1 EA相关蛋白,但在啮齿类中不识别。 Human ProIGF-I EA (92-105) antiserum was prepared by immunising rabbits with a 15 amino acid peptide derived from the E domain of human ProIGF-I EA linked to diphtheria toxoid. The peptide is located at the C terminus of the 153 amino acid ProIGF-I EA derived from alternative splicing of the human igf-1 gene. The antiserum recognises ProIGF-I EA but not ProIGF-I EB, ProIGF-I EC, mature IGF-I or IGF-II. Sequence analysis suggest it is likely to recognise ProIGF-I EA related proteins in a range of other species including non-human primates, rabbit, guinea-pig, dog, horse, sheep and goat but not rodents, due to alternative splicing of the igf-1 gene.
GroPep Bioreagents公司总部位于澳大利亚,成立于1990年,致力于向广大科研院所、生物技术及制药公司开发、生产和销售细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂,现有产品超过100多个,同时在世界范围内均设有分销商。公司在分子生物领域、发酵学及重组蛋白和单抗制备方面具有丰富的经验,在制造工艺方面,拥有大量专利技术,GroPep目前已通过AS/NZS ISO 9001:2016质量管理体系认证。