Rho Activator I(Cat. # CN01) is useful for efficient activation of RhoA, RhoB, and RhoC in a variety of cultured cells. The reagent activates Rho proteins in fibroblasts, neurons, epithelial, endothelial, and hematopoietic cells as well as other primary and immortalized lines. Cells treated with the activator can be subjected to any one of a number of assays that indicate an increase in Rho activity, including focal adhesion or stress fiber staining (Cat. # BK005) and Rho activity assays by G-LISA™ (Cat. # BK124). See Figure 1 for example of Rho activation measured by the G-LISA assay.
