1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA. The 1 kb and 3 kb bands contain more DNA to provide internal orientation. Ready-to-Use Ladders are provided pre-diluted in 1X loading buffer at a concentration of 20 ng/uL. A separate vial of 6X loading buffer is provided to add to your DNA samples. The loading buffer provided contains density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.
产品描述
1 kb DNA Ladder适用于测定250 bp至10 kb的线性双链DNA片段的大小。梯状条带由PCR和双链DNA的限制性酶消化产生的13个条带组成。Ikb和3 kb条带含有更多的DNA以提供内部取向。即用型阶梯在1X上样缓冲液中以20 ng/uL的浓度预稀释。提供一个单独的6X上样缓冲液小瓶以添加到您的DNA样本中。所提供的上样缓冲液含有密度剂和两种蓝色电泳示踪染料，其在1%琼脂糖凝胶中以约1.5kb和200 bp运行。nbsp;
