10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H2O2 is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.
Detect HRP in immunoassays
Detect trace amounts of hydrogen peroxide (H2O2)
Detect using fluorometer or microplate reader
λEx/λEm(pH 9)= 571/585 nm (end product)
ε= 54,000 (end product)
White to off-white solid soluble in DMSO or DMF
Store desiccated at -20°C and protect from light
C14H11CNO4
MW: 257.24
[119171-73-2]
Amplex Red is a registered trademark of Thermo Fisher Scientific.
产品描述
10-乙酰基-3，7-二羟基吩恶嗪（N-乙酰基-3，7-二羟基吩恶嗪或ADHP），其已经以名称Amplex销售®红色被认为是过氧化物酶的最佳荧光底物，因为它具有高度特异性和稳定性。底物本身在被H氧化前几乎是无色无荧光的 2 O 2在辣根过氧化物酶（HRP）的存在下，将所述化合物（以1：1化学计量反应）转化为高度红色荧光试卤灵。H 2 O 2由许多酶促反应产生，因此ADHP可用于检测许多不同酶的活性。
