SUMOylation of a target protein is a recently discovered post-translational modification that has been shown to play a critical role in protein
structure and function. Recent proteomic studies have identified hundreds of proteins that are SUMOylated. As SUMO modifications are
relatively new compared to other PTMs, tools to investigate SUMO are not well established. Appropriate lysis buffers, high quality affinity
reagents, and appropriate inhibitors are all critical reagents for effectively studying SUMO modifications. Here we describe a newly developed
Signal-Seeker SUMO 2/3 detection kit (BK162) that rapidly identifies SUMO 2/3 modifications for any target protein. This kit utilizes
optimized buffers and inhibitors to preserve SUMO 2/3 modifications unlike conventional, non-denaturing lysis buffers. Additionally, the
sensitivity of the kit allows for investigation of endogenous and dynamic changes of SUMO 2/3 modifications. Finally, we show that the
BK162 kit allows for SUMO 2/3 detection from both cell culture and tissue. Collectively, these studies highlight the utility of the BK162 kit
to investigate SUMO 2/3 modifications of several target proteins in various applications.
