The RalA G-LISA™ kit contains a Ral-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Ral is captured by this protein while inactive GDP-bound Ral is removed following washes. The active RalA bound to the wells is detected with a RalA specific antibody. The degree of RalA activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
