PanProbes™ One-Step RT-qPCR Kit delivers high sensitivity of the target RNA level due to its RScript reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency. The Universal qPCR Master Mix is a 2x concentrated, ready-to-use master mix optimized for probebased real-time PCR and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). It contains antibody-mediated hot-start Taq DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers and essentials for a success PCR reaction.