该产品别称为Phospho-sure,应用类型为:IP,WB,Please download the protocol below for detailed instructions on how to use Phospho-Sure in neuronal and other soft tissues.
产品描述
Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis.
Phospho-Sure™RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.