EvaGreen® 20 X水溶液，1 mL-EvaGreen-荧光染料-艾美捷科技有限公司

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中文名称: EvaGreen® 20 X水溶液，1 mL

英文名字: EvaGreen

供应商: Biotium

产品货号: 【特价品目】BTM-31000-T

产品报价: ￥询价/1ML

产品说明书: 点击查看

购买方式: 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料: EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer. The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis. See our ready-to-use Forget-Me-Not™ EvaGreen® qPCR master mixes for sensitive, competitively-priced qPCR reagents. We also offer Thiazole Green, 1000X in DMSO, which is identical to SYBR® Green I. EvaGreen dye and applications are covered by granted US and international patents.
Features:
Low PCR inhibition Exhibits much less PCR inhibition than SYBR® Green I via a smart "release-on-demand" DNA-binding technology Highly Sensitive Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals Nonmutagenic and noncytotoxic Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes Compatible with fast PCR protocols Minimal interference to PCR makes it possible to significantly shorten the chain extension time Compatible with multiplex PCR No dye migration from amplicon to amplicon when used at the recommended concentration Unsurpassed thermal stability, chemical stability and photostability No detectable dye decomposition in PCR buffer at 95-100

产品描述: EvaGreen®染料是一种绿色荧光核酸染料，其特征使得该染料可用于多种应用，包括qPCR和DNA熔解曲线分析、嗜热解旋酶依赖性扩增（tHDA）的实时监测和毛细管凝胶电泳。染料本身基本上是非荧光的，但在与dsDNA结合时变得高度荧光。DNA结合的染料具有与荧光素（FAM）或SYBR非常接近的激发和发射光谱®绿色I，使其易于与配备有488 nm氩激光器或具有该区域波长的任何可见光激发的仪器兼容。EvaGreen®染料在热和水解方面都非常稳定，在常规处理过程中提供了便利。EvaGreen®与SYBR不同，染料不渗透细胞膜，因此无致突变性和细胞毒性®绿色I，它迅速进入细胞，是一种强大的突变增强剂。nbsp; EvaGreen的独特性能®染料使其在定量实时PCR（qPCR）应用中特别有用。与广泛使用的SYBR相比®绿色I，EvaGreen®染料通常对PCR的抑制性较小，并且不太可能引起非特异性扩增。因此，EvaGreen®染料可以以比SYBR高得多的染料浓度使用®绿色I，产生用于液滴数字PCR、实时PCR和熔解曲线分析的高分辨率信号。nbsp; 查看我们的即用型勿忘我™EvaGreen®qPCR主混合液，用于灵敏、价格有竞争力的qPCR试剂。我们还提供噻唑绿色，1000 X的DMSO溶液，与SYBR相同®绿色岛& nbsp; EvaGreen染料及其应用已获得美国和国际专利。nbsp;
产品特点：
低PCR抑制 显示出比SYBR低得多的PCR抑制® 绿色I通过智能“按需释放”DNA结合技术 高灵敏度 染料的低PCR抑制允许较高的染料浓度用于大得多的qPCR和熔解曲线分析信号 无致突变性和无细胞毒性 标准艾姆斯试验无致突变性和细胞毒性; 完全不透细胞膜 与快速PCR方案兼容 对PCR的最小干扰使得可以显著缩短链延伸时间 与多重PCR兼容 当以推荐浓度使用时，扩增子之间无染料迁移 卓越的热稳定性、化学稳定性和光稳定性 在PCR缓冲液中在95-100°C下持续48小时没有可检测的染料分解; 在碱性或酸性条件下高度稳定; 可承受反复冻融循环 光谱与SYBR相似®绿色岛 兼容所有主要品牌的qPCR仪器和酶系统

产品形式: 该EvaGreen® 20 X水溶液，1 mL产品形式待更新请参考产品说明书

保存建议: 室温

其他: Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------中外知名首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq热启动酶和CF系列蛋白质标记染料等。 n目前，Biotium已将其许多专利技术授权给一些主要的国际生物技术公司，包括安捷伦、BD生物科学、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。