WB; Western Blotting (0.5 – 2 µg/mL). High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results.
Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Synthetic peptide immunogen, AKASPV[pY]LDILG
来源宿主
兔
反应性
人. Other species not yet tested but predicted to react with 小鼠, 大鼠, 鸡 TrkB-pY816/817 based on amino acid homology at the site.