WB; IH(P), Paraffin embedded; WB, IHC. Typical working dilution for IHC is 1:200 to 1:1,000 depending on tissue and detection method. For WB, a dilution range of 1:1,000 to 1:2,000 is recommended. GPx-4 exists as a tetramer and can reform into multimeric complexes even under reduced conditions. The reported molecular weight of the GPx-4 monomer is 22 kDa but is reported to rapidly form oligomers, thus higher molecular weight bands greater than 22 kDa are expected. Mitochondrial preparations are also recommended to enhance signal. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (VNYTQLVDLHARYAEC) corresponding to the amino acids 78-93 of human glutathione peroxidase 4 (Isoform Mitochondrial) conjugated to KLH has been used as the immunogen. Human, rat and mouse sequences are identical.
