Prostaglandin F2alpha (PGF2alpha) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. It is produced in various tissues. It contracts smooth muscle and has been used to induce labor. It has a luteolytic effect and has been considered a luteolytic hormone.Kit Components:P9053-35E EIA Buffer: 1x30ml. To be used to dilute enzyme conjugate and PGF2a standards.P9053-35EF Wash Buffer (10X): 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.P9053-35G Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.P9053-35H Extraction Buffer (5X): 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.P9053-35J Prostaglandin F2a (HRP): 1x150ul. PGF2a HRP concentrate. Blue capped vial.P9053-35K Prostaglandin F2a Standard: 1x100ul. PGF2a standard at the concentration of 1mg/ml. Green capped vial.P9053-35L Microtiter Plate: 1x96 wells microplate with anti-PGF2a rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
