Norgen’s Sputum Liquification Buffer is designed for the liquification of viscous sputum samples prior to isolation of cells for microscopy or nucleic acids for molecular testing. The homogenization of sputum samples is often desirable, particularly when there is a requirement for centrifugation to pellet cells or prior to the isolation of nucleic acids. The Sputum Liquification Buffer contains the mucolytic agent dithiothreitol which acts by reducing the disulfide bonds of the mucus proteins, thereby decreasing sample viscosity and allowing for easy handling and processing. Genomic DNA or RNA can then be isolated from the homogenized sample as Norgen’s Sputum Liquification Buffer is both DNAse and RNAse-free.
