- 中文名称
Neonatal Cardiomyocyte Isolation System, BioAssay Kit
- 英文名字
- Neonatal Cardiomyocyte Isolation System, BioAssay Kit
- 供应商
- USBiological
- 产品货号
- N1057
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Neonatal Cardiomyocyte Isolation System has been introduced to provide researchers with a reliable, convenient, and consistent cell isolation system. By utilizing purified rather than crude enzyme preparations, it has been possible to minimize the lot-to-lot variation. In addition, United States Biological use-tests the kits by isolating cardiomyocytes from neonatal rat hearts to assure performance, reliability and consistent yield of viable cells. The kit has been formulated in conjunction with Dr. Ronal MacGregor. The method is based on that described by Toraason, et al. (1988) in which the minced tissue is incubated overnight with purified trypsin at 4°C. As pointed out by Toraason, this step reduces the hands-on time required to harvest cells compared to the time involved in sequential incubations in warm trypsin or collagenase. Also, purified collagenase is utilized to maximize yield and viability. This kit contains sufficient materials for five separate tissue dissociations, each containing up to twelve hearts. For larger or smaller tissue samples prepare proportionate volumes ofreagents at each step and combine them in the same ratio as described in the protocol.Kit Components:N1057-01: Hank's Buffer, 1x500ml Sterile calcium- and magnesium-free Hank's Balanced Salt Solution, pH 7.4. The solution is used for reconstituting N1057-02 Trypsin and N1057-03 Inhibitor in addition to serving as the medium for the dissociation.N1057-02: Trypsin Vials, 5x1000ug, 3X crystallized, dialyzed against 1mM HCl, filtered through 0.22um pore size membrane, and lyophilized. Reconstitute with 2ml N1057-01 Hank's Buffer and swirl gently to dissolve contents. Store at 4°C.N1057-03: Inhibitor Vials, 5x2000ug Supplied as a 0.22um membrane filtered, lyophilized powder. Reconstitute with 1ml N1057-01 Hank's Buffer and swirl gently to dissolve contents. Store at 4°C.N1057-04: Collagenase Vials 5x1500 Units Supplied as a 0.22um membrane filtered, lyophilized powder which has been chromatographically purified. It contains <50 caseinase units/mg and is composed of two separable but very similar collagenases. Reconstitute with 5ml prepared N1057-05 L-15 Media Powder and swirl gently to dissolve contents. Store at 4°C.N1057-05: L-15 Media Powder, 1x1L Reconstitute entire contents of pouch by cutting open top of envelope and pouring contents into beaker containing 800ml of cell culture grade water. Rinse pouch 2-3 times with additional 100ml. Bring total volume to 1 liter and filter through a 0.22um filter.N1057-06: Cell Strainers (Falcon), 5 and a card correlating phenol red color with pH for checking the pH of balanced salt solution and culture medium.Storage and Stability:May be stored at 4°C. Stable for 6 months.
- 产品特点
- 针对Neonatal Cardiomyocyte Isolation System, BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Neonatal Cardiomyocyte Isolation System, BioAssay Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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