Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.
产品描述
测量二酰基甘油（DAG）信号动力学，筛选Gq活性的GPCR，以及更多的活细胞荧光检测。将DAG传感器添加到细胞中，30小时后在自动酶标仪或荧光成像系统上测量稳健的信号。增加的红色荧光指示DAG增加。这些检测试剂在BacMam中进行基因编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率，以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括DAG传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（hM 1 R BacMam和25 mM卡巴胆碱水溶液）。10 mL大小也是可用的。其他Montana Molecular工具，包括BacMam包装的GPCR，可以很容易地与该测定共表达。
