Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - D1 Dopaine Receptor (D1R) assay. Add the unmodified D1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, D1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
产品描述
用活细胞荧光Borealis arrestin-D1多巴胺受体（D1 R）测定法真实的实时测量arrestin信号传导动力学。 将未修饰的D1受体和Borealis传感器添加到细胞中，并在第二天在自动平板阅读器或荧光成像系统上测量稳健的信号。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括抑制蛋白传感器BacMam、D1 R BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（AT 1 R BacMam和5 mM血管紧张素II水溶液）。还提供10毫升和60毫升规格，以及适用于其他几种受体的Borealis试剂盒。其他Montana Molecular工具（包括cADDis cAMP传感器）可与该测定一起使用。
