Measure real-time cAMP signaling kinetics in the nucleus with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.
产品描述
用这种活细胞荧光测定法测量细胞核中的实时cAMP信号传导动力学。将NLS标记的cADD添加到您的细胞中，并在第二天在荧光成像系统上测量强大的信号。减少的绿色荧光指示cAMP增加。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括cAMP传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（B2 AR BacMam和10 mM异丙肾上腺素HCl溶液）。30 mL大小也是可用的。其他Montana分子工具，包括BacMam包装的GPCR、红色荧光膜靶向cAMP传感器或DAG或钙的红色荧光传感器，可以很容易地与该测定共表达。
