- 中文名称
Histamine, BioAssay ELISA Kit
- 英文名字
- Histamine, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- H5085-20
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Histamine is a biogenic amine, which is formed by enzymatical decarboxylation from the amino acid histidine. It occurs in the mast cells and basophilic white cells bound to heparin. In the course of a type I allergy, endogenic histamine from the mast cells and basophilic leukocytes is released after antigen binding to membrane-associated IgE, and typical allergical reactions appear.But histamine can also enter the human body via the nutrition and can thus cause pseudoallergic food intolerances. The appearing symptoms are not distinguishable from those of a real allergy. The term 'food allergy' is however only used, if the pathologic symptoms develop in the course of an immunological reaction. Non-immunological reactions to food are defined as food intolerances. Food intolerances, which are caused by increased histamine concentrations, are clinically characterized by rash, diarrhoea, vomiting, nausea, itching, headache and asthma. The extent of the reaction is dependent on the ingested amount of histamine. These reactions can be enhanced by a lack of diaminoxidase.Separated from histamine intolerances, toxic reactions exist, which are caused by very high histamine concentrations. Toxic histamine concentrations, which can cause the so-called scombroid reaction, appear after bacterial degradation of protein-rich food, especially fish. Histamine is found above all in cheese, fish, spinach, smoked products and red wine. The histamine concentrations in different types of food, but also in the same food show frequently great differences. In different types of cheese there can e.g. be found histamine contents between 600 and 1000mg/kg. Histamine ELISA is a quick, economical and sensitive method to detect histamine in wine, fish or cheese. After an appropriate sample preparation, 40 samples can be tested in duplicate within 80 minutes.Intended Use:For the quantitative determination of Histamine in food.Sensitivity: 2ng/mLRecovery (wine, fish, cheese): ~90%Incubation Time: 80 minTest Principle:Histamine, Food, BioAssay ELISA Kit quantitative test is based on the principle of the enzyme linked immunosorbent assay. A histamine conjugate is bound on the surface of a microtiter plate. Samples or standards containing derivatized histamine and an antibody directed against histamine are given into the wells of the microtiter plate. Immobilized and free histamine compete for the antibody binding sites. After 30 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate directed against the histamine antibody is given into the wells and after another 30 minutes incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of histamine is indirectly proportional to the color intensity of the test sample.Kit Components:1. Microtiter plate 1x96 wells each, coated with histamine conjugate.2. Standard 4ng/ml 1x4ml each.3. Standard 2ng/ml 1x4ml each.4. Standard 4ng/ml 1x4ml each.5. Standard 10ng/ml 1x4ml each.6. Standard 40ng/ml 1x4ml each.7. Standard 100ng/ml 1x4ml each.8. Reaction Solution: 1x3ml, ready to use.9. Neutralizing Solution: 1x15ml, ready to use.10. Anti-Histamine Antibody (mouse): 1x6ml, ready to use.11. Conjugate (anti-mouse-IgG-HRP): 1x15ml, ready to use.12. Substrate Solution (TMB): 1x15ml, ready to use.13. Stop Solution (0.5 M H2SO4): 1x15ml, ready to use.14. Sample Diluent (PBS): 1x60ml, ready to use.15. Washing Solution 10x (PBS + Tween 20): 1x60ml.Dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Histamine, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histamine, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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