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细胞分析试剂盒
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中文名称

Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)

英文名字
Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)
供应商
USBiological
产品货号
G8124-11
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
United States Biological has improved the standard GSH/GSSG Assay Kit by using a new and improved GSH scavenger.Reduced glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) that contains a free thiol group. GSH is a major tissue antioxidant that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water:H2O2 + 2GSH ---------> GSSG + 2H2O (catalyzed by GPx)In the GPx catalyzed reaction, the formation of a disulfide bond between two GSH molecules gives rise to oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of beta-nicotinamide adenine dinucleotide phosphate (beta-NADPH):GSSG + NADPH -----------> 2GSH + NADP+ (catalyzed by GR)When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. Therefore, the determination of the GSH/GSSG ratio and the quantification of GSSG are useful indicators of oxidative stress in cells and tissues.Kit Components:G8124-11A: Assay Buffer, 2X, 1x125ml. General buffer used to dilute samples and reagents*G8124-11B: GSSG Standard (10uM), 1x2ml.G8124-11C: Scavenger, 1x3ml. Thiol scavenger to keep GSSG in oxidized form.G8124-11D: DTNB, 4x1vial. Lyophilized 5,5'-dithiobis-2-nitrobenzoic acid.G8124-11E: MPA, 5%, 1x40ml. Metaphosphoric acid used to deproteinate samples.*G8124-11F: Glutathione reductase, 1x130ul. Solution containing recombinant glutathione reductase.*G8124-11G: NADPH, 4x1vial. Lyophilized beta-nicotinamide adenine dinucleotide phosphate.Storage and Stability:Store *G8124-11B, *G8124-11F and *G8124-11G at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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