FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate.
产品描述
Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio. FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
产品形式
Liquid - clear, colorless, odorless
保存建议
Store Chemiluminescent Substrate at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
