The trimethoprim-PEG3-amine (TMP-PEG3-amine) can be conjugated to various probes to make trimethoprim-based chemical tag (TMP-tag). The TMP-tag is based on the high affinity (Kd ~ 1 nM) binding of TMP to the E.coli dihydrofolate reductase (eDHFR). The target protein, fused to eDHFR via standard molecular cloning technology, is labeled by the TMP-probe in a non-covalent manner. The low affinity of TMP with mammalian DHFR (Kd > 1 uM) ensures minimal background. The TMP-tag has been demonstrated to enable protein visualization and manipulation of both intracellular and surface targets in mammalian cells. The TMP-PEG-amine can be used in combination with our Mix-n-Stain™ Small Ligand Labeling Kits to generate fluorescent chemical tags without purification for cell surface or intracellular staining.
产品描述
甲氧苄啶-PEG 3-胺（TMP-PEG 3-胺）可以与各种探针缀合以制备基于甲氧苄啶的化学标签（TMP-标签）。TMP标签基于高亲和力（K d 约InM）TMP与细胞的结合。 E.coli二氢叶酸还原酶（eDHFR）。通过标准分子克隆技术与eDHFR融合的靶蛋白由TMP探针以非共价方式标记。TMP与哺乳动物DHFR（K d>1 μ M）确保最小的背景。TMP标签已被证明能够实现哺乳动物细胞中的细胞内和表面靶标的蛋白质可视化和操纵。nbsp; TMP-PEG-胺可以与我们的Mix-n-Stain™小配体标记试剂盒组合使用，以产生荧光化学标签，而无需纯化用于细胞表面或细胞内染色。
