DiOC16(3) is a lipophilic cyanine dye with two C16 hydrocarbon chains. It can be optimally excited by the 488 nm argon laser line with green fluorescence emission (&lamda;Ex/&lamba;Em = 484/501 nm). The dye can be used to stain cytoplasmic membranes. Recently, DiOC16 has been combined with DPA to detect cell membrane potential change with high sensitivity. Please also see the DiO/DPA Membrane Potential Detection Kit (30037)).