Dilinoleyl DiO (1,1'-dilinoleyl-3,3'-oxacarbocynanine perchlorate), also known as FAST DiO, has identical absorption and emission spectra as DiO. However, due to its unsaturated hydrocarbon chains, dilinoleyl DiO has a much faster lateral diffusion rate in cell membranes than DiO.
&lamba;Ex/&lamba;Em (MeOH) = 484/499 nm
ε = 140,000
Yellow orange solid soluble in ethanol, DMF or DMSO