D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts. For real-time detection of caspase-3 activity in intact cells, see NucView® Caspase-3 Substrates.
Ex/Em of end product (R110) = 496/520 nm
Off-white to pink solid soluble in DMSO
Store at 4°C and protect from light
C32H26F6N4O13
MW: 788.57
产品描述
D 2R（罗丹明110，双-（L-天冬氨酸酰胺），二三氟乙酸盐）是半胱天冬酶（包括半胱天冬酶3和7）的一般底物。与其他半胱天冬酶的荧光底物不同，D 2R似乎是膜渗透性的，因此可以用于在没有渗透压休克的情况下测量活细胞中的胱天蛋白酶活性，用于流式细胞术研究以及细胞提取物。完整细胞中胱天蛋白酶-3活性的实时检测见NucView®胱天蛋白酶-3底物。
