Oxidative Stress/ Damage->DNA / RNA Damage and Repair->UV Induced DNA Damage Kits
应用类型
Our OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
免疫原
Measure CPD structures of DNA lesions induced by UV light
来源宿主
OxiSelect™ 细胞紫外线诱导 DNA 损伤染色套件(CPD)(OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit (CPD))保存建议,请查看该产品的使用手册