HGF 重组蛋白-HGF Recombinant Protein代理/参数/厂家-重组蛋白-艾美捷科技有限公司

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背景资料: HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF, sourced from HEK293 cells, is a 79.4 kDa polypeptide consisting of 695 amino acid residues. As a result of glycosylation, Recombinant Human HGF migrates with an apparent molecular mass of approximately 68-85 kDa by SDS-PAGE gel, under non-reducing conditions.

序列: alpha chain: KRRNTIHEFK KSAKTTLIKI DPALKIKTKK VNTADQCANR CTRNKGLPFT CKAFVFDKAR KQCLWFPFNS MSSGVKKEFG HEFDLYENKD YIRNCIIGKG RSYKGTVSIT KSGIKCQPWS SMIPHEHSFL PSSYRGKDLQ ENYCRNPRGE EGGPWCFTSN PEVRYEVCDI PQCSEVECMT CNGESYRGLM DHTESGKICQ RWDHQTPHRH KFLPERYPDK GFDDNYCRNP DGQPRPWCYT LDPHTRWEYC AIKTCADNTM NDTDVPLETT ECIQGQGEGY RGTVNTIWNG IPCQRWDSQY PHEHDMTPEN FKCKDLRENY CRNPDGSESP WCFTTDPNIR VGYCSQIPNC DMSHGQDCYR GNGKNYMGNL SQTRSGLTCS MWDKNMEDLH RHIFWEPDAS KLNENYCRNP DDDAHGPWCY TGNPLIPWDY CPISRCEGDT TPTIVNLDHP VISCAKTKQL R beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS

保存建议: The recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

其他: ProSci Incorporated于1998年成立于美国加州圣地亚哥市,从事抗体等科研试剂的研发、生产和销售。产品涵盖了细胞凋亡、信号转导、免疫、肿瘤、细胞生物、神经生物、传染病等领域。ProSci拥有超过30000种单克隆抗体和多克隆抗体。ProSci的抗体大都经免疫亲和层析纯化,具有高纯度、高亲和力、特异性强等特点,所有的ProSci抗体都经过了严格的验证测试和质量控制,已保证高质量的产品。此外,ProSci还提供标记二抗,重组蛋白,细胞和组织裂解液,细胞涂片,组织切片,多肽等产品与抗体配套使用。