Purification and enrichment of intact urinary exosomes for functional studies; Bind and elute all RNA irrespective of size or GC content, without bias; Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile urine input volume (11 mL to 30 mL); No phenol extractions, Proteinase K treatment, nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes are required; No precipitation reagents nor overnight incubation required; Compatible with urine from any species; Pure exosomes are purified and are free-from any other RNA-binding proteins.