血浆/血清无细胞循环和病毒核酸纯化大提试剂盒（提取试剂盒）-Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit-细胞分析试剂盒-艾美捷科技有限公司

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英文名字: Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit

产品描述: This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating including exosomal RNA as well as viral DNA/RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate from volumes ranging from 2 mL to 5 mL. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum circulating and viral nucleic acid is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

产品特点: Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution; Versatile plasma/serum input ranging from 2 mL to 5 mL; No phenol extractions; No carrier RNA; Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating DNA, circulating and exosomal RNA, viral DNA and viral RNA into a flexible elution volume ranging from 50 µL to 100 µL; Purify high-quality RNA/DNA in 35 - 40 minutes; Compatible with Streck Cell-Free DNA BCT® Tubes.

其他: Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。