Plasmid pEGFP-HMGB1 was obtained by inserting the coding sequence for the rat HMGB1 into pEGFP-N1 (Clontech) using the EcoRi and SacII restriction sites (Scaffidi et al. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature 2002, 418: 191-5). It expresses HMGB1-GFP, which is rat HMGB1 (at the N terminus) fused to GFP (at the C terminus). This protein is fully functional and is functionally equivalent to unmodified HMGB1 (Pallier et al. Association of chromatin proteins HMGB1 and HMGB2 with mitotic chromosomes. Mol Biol Cell 2003, 14: 3414-26).
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HMGBiotech是一家由HMGB领域的领先科学家Marco E. Bianchi创立的生物技术公司。nHMGBiotech是HMGB53质量控制氧化还原变体的唯一生产商。该公司为活跃在这一有前途的领域的其他研究人员和公司提供试剂、服务和想法，并帮助Bianchi实验室（the Bianchi lab）开发检测和试剂。
