This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amylin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amylin and unlabeled Amylin (Standards or samples) with the pre-coated antibody specific to Amylin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amylin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amylin in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该牛肿瘤坏死因子α(TNFa)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
