This assay has high sensitivity and excellent specificity for detection of Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7). No significant cross-reactivity or interference between Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人信号淋巴细胞激活分子家族,成员7(SLAMF7)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.