This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4). No significant cross-reactivity or interference between Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 5 (MCM5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 5 (MCM5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠蛋白磷酸酶2A激活剂，调节亚基4(PPP2R4)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
