This assay has high sensitivity and excellent specificity for detection of Heparanase (HPSE). No significant cross-reactivity or interference between Heparanase (HPSE) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Asymmetrical Dimethylarginine (ADMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Asymmetrical Dimethylarginine (ADMA) and unlabeled Asymmetrical Dimethylarginine (ADMA) (Standards or samples) with the pre-coated antibody specific to Asymmetrical Dimethylarginine (ADMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠乙酰肝素酶(HPSE)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
