This assay has high sensitivity and excellent specificity for detection of Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). No significant cross-reactivity or interference between Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Orexin A (OXA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Orexin A (OXA) and unlabeled Orexin A (OXA) (Standards or samples) with the pre-coated antibody specific to Orexin A (OXA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Orexin A (OXA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Orexin A (OXA) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠内分泌腺源性血管内皮生长因子(EG-VEGF)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.