This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Malondialdehyde (MDA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Malondialdehyde (MDA) and unlabeled High Sensitive Malondialdehyde (MDA) (Standards or samples) with the pre-coated antibody specific to High Sensitive Malondialdehyde (MDA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Malondialdehyde (MDA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Malondialdehyde (MDA) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠脑源性神经营养因子(BDNF)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.