- 中文名称
Cortisol, Saliva, Human, BioAssay ELISA Kit
- 英文名字
- Cortisol, Saliva, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C7904-22
- 产品报价
- ￥1/1x96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations , physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison's and Cushing's diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1-2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva , salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.Intended Use:This kit is intended for the determination of cortisol in human saliva by competitive ELISA. Principle of the Test:The assay is carried out following a typical competitive ELISA format. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labeled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of cortisol in the sample. A set of standards is used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.Detection Range:1-100ng/mlSensitivity:1.0ng/mlIntra-Assay: CV <11% Inter-Assay: CV <10%Kit Components:C7904-22A: Microtiter Strip, 1x96 wells. Coated with polyclonal antibody against cortisol. C7904-22B: Cortisol (HRP), 50X, 1x300ul. Cortisol-HRP conjugate in a protein-based buffer with a non-mercury preservative. Preparation: Dilute 1:50 in assay buffer before use (eg. 40ul of HRP in 2 ml of assay buffer). If the whole plate is to be used dilute 240ul of HRP in 12ml of assay buffer. Discard any that is left over.*C7904-22C1: Low Control, 11.1(7.7-12.8ng/ml) 1x600ul*C7904-22C2: High Control, 54.4(38-63.3ng/ml)1x600ul Cortisol in a protein-based buffer with a non-mercury preservative. Prepared by spiking buffer with a defined quantity of cortisol. Refer to vial label for expected value and acceptable range. Stability: Once opened, the control should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles. *C7904-22D1: Calibrator A, 0ng/ml cortisol, 1x2ml*C7904-22D2: Calibrator B, 1ng/ml cortisol, 1x600ul*C7904-22D3: Calibrator C, 3ng/ml cortisol, 1x600ul*C7904-22D4: Calibrator D, 10ng/ml cortisol,1x600ul*C7904-22D5: Calibrator E, 30ng/ml cortisol, 1x600ul*C7904-22D6: Calibrator F, 100ng/ml cortisol, 1x600ulNote: Once opened, the calibrators should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles.C7904-22E: Wash Buffer, 10X, 1x50mlPreparation: Dilute 1:10 in distilled or deionized water before use. If the whole plate is to be used dilute 50ml of the wash buffer concentrate in 450ml of water.C7904-22F: Assay Buffer, 1x15ml. Ready to use. Protein-based buffer with a non-mercury preservative.C7904-22G: TMB Substrate, 1x16ml. Ready to use. Tetramethylbenzidine and hydrogen peroxide in a non-DMF or DMSO containing buffer.C7904-22H: Stop Solution, 1x6ml. Ready to use. 1M sulfuric acid. Caution: Corrosive! Storage and Stability:Store *C7904-22C and *C7904-22D at 4°C until opened. Once opened aliquot and store at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Cortisol, Saliva, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Cortisol, Saliva, Human, BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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