The mouse Interleukin (IL-4) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-4 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-4 concentration are added to these wells and allowed to complex with the bound IL-4 antibody. A biotinylated mouse IL-4 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-4 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
产品特点
灵敏度:7.8 pg/mL - 500 pg/mL。此外该ELIAS试剂盒包含：2 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
