Sulfonamides are widely used in animal industry and play an important role in controlling and treatment livestock and poultry diseases. Sulfonamides have become a threat for human health and affected the export of animal derived food due to Sulfonamides residues for abuse，not abiding by withdrawal time. Sulfonamides residues limit is 100mg/kg according to No.235 document of the Ministry of Agriculture of the People's Republic of China. The method of instrumental analytical is major to detect Sulfonamides residues，but it needs expensive instruments，professional operators and complex pre-treatment; While the method of enzyme linked immunoassay has advantages with simple，rapid，high sensitivity，good specificity and low cost. This kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Tissue （Chicken，porcine，liver），porcine urine，milk，etc.Intended Use：This BioAssay kit allows for the in vitro quantitative determination of total sulfonamides concentrations in milk，tissue （chicken，porcine，liver），porcine urine.Sensitivity：2ppbRange：2-162ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Sulfonamides antigen. Standards or samples are added to the appropriate microtiter plate wells with Sulfonamides specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Sulfonamides and Sulfonamides in standards or samples with the Sulfonamides special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Sulfonamides in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate （96T），96x wellsAssay plate （48T），48x wellsStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （5x） （96T），1x20mlSample DIluent （5x） （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
