中文名称

原儿茶酸二加氧酶（25mg）

英文名字

Protocatechuate Dioxygenase

供应商

Cayman Chemical

产品货号

10011353-25

产品报价

￥已停产

产品说明书

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购买方式

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产品新闻

背景资料

Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2.{15534} The PCD holoenzyme consists of α (23 kDa) and β (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.{15536} Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex.{15534} PCD is an important component of bacterial metabolism of aromatic compounds found in the soil. However, many publications are now reporting the usefulness of the PCD/PCA oxygen scrubbing (oxygen scavenging) system for maintaining anaerobic experimental conditions.{15535,15565} Cayman’s protocatechuate dioxygenase is the 3,4-PCD expressed and purified from a bacterial overexpression system. 3,4-PCD catalyzes the oxidative ring cleavage of 3,4-dihydroxybenzoate to produce β-carboxy-cis,cis-muconate. Activity of the PCD can be measured by monitoring the rate of O2 uptake with an oxygen electrode, or by measuring the amount of product (β-carboxy-cis,cis-muconate) formed at 290 nm.

产品描述

提供的原儿茶酸二加氧酶（25mg）产品形式为：A lyophilized powder from a solution of 20 mM potassium phosphate, pH 7.2, containing 100 mM sodium chloride.

产品形式

Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2.{15534} The PCD holoenzyme consists of �� (23 kDa) and �� (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.{15536} Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex.{15534} PCD is an important component of bacterial metabolism of aromatic compounds found in the soil. However, many publications are now reporting the usefulness of the PCD/PCA oxygen scrubbing (oxygen scavenging) system for maintaining anaerobic experimental conditions.{15535,15565} Cayman��s protocatechuate dioxygenase is the 3,4-PCD expressed and purified from a bacterial overexpression system. 3,4-PCD catalyzes the oxidative ring cleavage of 3,4-dihydroxybenzoate to produce ��-carboxy-cis,cis-muconate. Activity of the PCD can be measured by monitoring the rate of O2 uptake with an oxygen electrode, or by measuring the amount of product (��-carboxy-cis,cis-muconate) formed at 290 nm.

保存建议

建议您在收到Cayman Chemical品牌的原儿茶酸二加氧酶（25mg）产品后置于-80度保存，产品有效期为180天.

其他

Cayman Chemical向全球科学工作者提供多研究领域的生化、免疫试剂和分析试剂盒，其产品被广泛应用于肿瘤、氧化氮、神经学、凋亡、氧化性损伤、内分泌学等不同研究领域.Cayman Chemical可提供用于检测的特色试剂盒，如：类花生酸类物质、游离的生物标志、环核苷、激素及氧化氮等；也提供多种高质量试剂，包括：类花生酸类物质、氧化氮试剂及许多相关脂质、脂肪酸、酶和抗体.作为Cayman Chemical在中国的区域总代理，艾美捷科技有限公司将为中国客户提供最全面的Cayman Chemical产品以及客户订制化服务.

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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