Mouse Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. LIF signals through receptors gp190 (LIFR) and gp130 to activate STAT3 which ultimately promotes mouse embryonic stem cell self-renewal and pluripotentcy of long term cultures. This activity is similar to that attributed to FGF-basic (FGF-2) for human embryonic stem cells. Recombinant mouse LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 20 kDa.
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来源宿主
Other - E.coli
溶解建议
0.01M acetic acid
保存建议
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.