Calcium measurement is critical for numerous biological investigations。 Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy， flow cytometry， fluorescence spectroscopy and fluorescence microplate readers。 Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators。 However， Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis， and require harsh cell loading conditions to maximize their cellular calcium responses。 Fluo-8 dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm。 Fluo-8 AM only requires room temperature while Fluo-3 AM and Fluo-4 AM require 37 ?C cell loading。 In addition， Fluo-8 is 2 times brighter than Fluo-4 AM， and 4 times brighter than Fluo-3 AM。 AAT Bioquest offers a set of our outstanding Fluo-8 reagents with different calcium binding affinities （Fluo-8: Kd = 389 nM； Fluo-8H: Kd = 232 nM； Fluo-8L: Kd = 1。86 礛； Fluo-8FF: Kd = 10 礛）。 We also offer versatile packing sizes to meet your special needs， e。g。， 1 mg； 10x50 礸； 20x50 礸； HTS packages with no additional packaging charge。 ***Patents Pending***
