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Cytoskeleton小G蛋白&细胞成像产品线新春大促啦!

发布者:艾美捷科技    发布时间:2023-01-10     
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为答谢和回馈广大科研工作者们长期以来对武汉艾美捷科技有限公司的大力支持,在新春到来之际,艾美捷科技联合细胞骨架领域顶级供应商 Cytoskeleton 联合推出:小G蛋白&细胞成像产品线新春超值大促,一年仅此一次,千万不要错过啊!本次促销活动产品涵盖  小G蛋白活化检测试剂盒激动剂、和Tubulin,Actin,溶酶体,DNA,质膜等细胞组分成像的荧光染料等。  


活动详情:

时间:2023年1月15日-2023年3月15日

对象:仅限中国区终端客户

品目:Cytoskeleton小G蛋白&细胞成像相关产品(详情见下方活动品目列表)

内容: 直享 6.5折超低优惠!  


活动品目列表:

分类货号产品名称规格原价折后价
Actin抗体CSK-AAN02Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1)1x500ul4547 2955
CSK-AAN02-SAnti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1)1x125ul1634 1062
小G蛋白活化检测试剂盒CSK-BK001Actin Binding Protein Spin-Down Assay KitKIT30-100assays12423 8075
CSK-BK003Actin Polymerization Biochem KitKIT30-100assays14149 9197
CSK-BK008Ras Activation Assay Biochem KitKIT50assays11991 7794
CSK-BK008-SRas Activation Assay Biochem KitKIT20assays6073 3947
CSK-BK030Combo RhoA/Rac1/Cdc42 Activation Assay KitKIT3x10assays15798 10269
CSK-BK032-SArf1 Pulldown Activation Assay KitKIT20assays7290 4739
CSK-BK033-SArf6 Pulldown Activation Assay KitKIT20assays7290 4739
CSK-BK034Cdc42 Pulldown Activation Assay KitKIT50assays11991 7794
CSK-BK034-SCdc42 Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK035Rac1 Pulldown Activation Assay KitKIT50assays11991 7794
CSK-BK035-SRac1 Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK036RhoA Pulldown Activation Assay KitKIT80assays11991 7794
CSK-BK036-SRhoA Pulldown Activation Assay KitKIT20assays6073 3947
CSK-BK037G-Actin : F-Actin In Vivo Assay KitKIT30-100assays11930 7754
CSK-BK040RalA Activation Assay Biochem KitKIT50assays11991 7794
CSK-BK121RhoA G-LISA Activation Assay (luminescence)KIT96assays14920 9698
CSK-BK124RhoA G-LISA Activation Assay (colorimetric)KIT96assays14920 9698
CSK-BK124-SRhoA G-LISA Activation Assay (colorimetric)KIT24assays9001 5851
CSK-BK124-XLRhoA G-LISA Activation Assay (colorimetric)大规格询价询价
CSK-BK125Rac1,2,3 G-LISA Activation Assay Kit (colorimetric)KIT96assays14920 9698
CSK-BK126Rac1 G-LISA Activation Assay KitKIT96assays14920 9698
CSK-BK127Cdc42 G-LISA Activation Assay Kit (colorimetric)KIT96assays14920 9698
CSK-BK127-SCdc42 G-LISA Activation Assay Kit (colorimetric)KIT24assays9001 5851
CSK-BK128Rac1 G-LISA Activation Assay Kit (colorimetric)KIT96assays14920 9698
CSK-BK128-SRac1 G-LISA Activation Assay Kit (colorimetric)KIT24assays9001 5851
CSK-BK129RalA G-LISA Activation Assay Kit (colorimetric)KIT96assays14920 9698
CSK-BK131Ras G-LISA Activation Assay Kit (colorimetric)KIT96assays14920 9698
CSK-BK131-XLRas G-LISA Activation Assay Kit大规格询价询价
CSK-BK135G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) (colorimetric)3KITS(24assays/kit)24861 16160
CSK-BK150RhoA ELISA Kit (colorimetric)KIT96assays8138 5290
小G蛋白相关活化剂及抑制剂CSK-CN01-ARho Activator5x10units3406 2214
CSK-CN01-BRho Activator20x10units12423 8075
CSK-CN02-ARac and Cdc42 Activator5x10units3406 2214
CSK-CN02-BRac and Cdc42 Activator20x10units12423 8075
CSK-CN03-ARho activator II3x20ug3406 2214
CSK-CN03-BRho activator II9x20ug9602 6241
CSK-CN03-XLRho activator II大规格询价询价
CSK-CN04-ARho/Rac/Cdc42 Activator I3x20ug3406 2214
CSK-CN04-BRho/Rac/Cdc42 Activator I9x20ug9602 6241
CSK-CT03-AC3 transferase protein1x25ug3406 2214
CSK-CT03-CC3 transferase protein4x25ug11930 7754
CSK-CT04-ACell Permeable Rho Inhibitor (C3 Trans based))1x20ug3406 2214
CSK-CT04-BCell Permeable Rho Inhibitor (C3 Trans based))5x20ug10234 6652
CSK-CT04-CCell Permeable Rho Inhibitor (C3 Trans based))20x20ug26911 17492
Tubulin,Actin,溶酶体,DNA,质膜染料CSK-CY-SC001SiR-Actin Kit50-300slides8600 5590
CSK-CY-SC002SiR-Tubulin Kit50-300slides8600 5590
CSK-CY-SC006Cytoskeleton Kit (SiR-Actin + SiR-Tubulin)50-300slideseach14318 9307
CSK-CY-SC007SiR-DNA Kit50-300slides5456 3546
CSK-CY-SC012SiR-Lysosome Kit50-300slides6258 4067
CSK-CY-SC013SiR700-Actin Kit35-200slides8600 5590
CSK-CY-SC014SiR700-Tubulin Kit35-200slides8600 5590
CSK-CY-SC015SiR700-DNA Kit35-200slides5456 3546
CSK-CY-SC016SiR700-Lysosome Kit35-200slides6258 4067
CSK-CY-SC020Flipper TR membrane tension sensor35-200slides6504 4228
CSK-CY-SC101SPY505-DNA Probe100slides5456 3546
CSK-CY-SC201SPY555-DNA Probe100slides5456 3546
CSK-CY-SC202SPY555-Actin Probe100slides7306 4749
CSK-CY-SC203SPY555-Tubulin Probe100slides7306 4749
CSK-CY-SC204SPY555-BG Substrate, Abs./Em. 555/580 nm35nmoles5456 3546
CSK-CY-SC301SPY595-DNA Probe100slides5456 3546
CSK-CY-SC401SPY620-DNA Probe100slides5456 3546
CSK-CY-SC402SPY620-Actin Probe100slides7306 4749
CSK-CY-SC404SPY620-BG Substrate, Abs./Em. 619/635 nm35nmoles5456 3546
CSK-CY-SC501SPY650-DNA Probe100slides5456 3546
CSK-CY-SC503SPY650-Tubulin Probe100slides7306 4749
CSK-CY-SC504SiR650-BG Substrate, Abs./Em. 652/674 nm35nmoles5456 3546
CSK-CY-SC601SPY700-DNA Probe100slides5456 3546
CSK-CY-SC604SiR700-BG Substrate, Abs./Em. 696/718 nm35nmoles5456 3546
CSK-MG01-02MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499  em 507  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3560 2314
CSK-MG01-10MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499  em 507  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides6766 4398
CSK-MG02-02MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555  em 570  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3560 2314
CSK-MG02-10MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555  em 570  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides6766 4398
CSK-MG03-02MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580  em 620 50-300 slides  (MEMBRIGHT Family Probe)50-300slides3560 2314
CSK-MG03-10MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580  em 620  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides6766 4398
CSK-MG04-02MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650  em 673  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3560 2314
CSK-MG04-10MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650  em 673  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides6766 4398
CSK-MG05-02MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650  em 720  50-300 slides  (MEMBRIGHT Family Probe)50-300slides3560 2314
CSK-MG05-10MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650  em 720  250-1500 slides  (MEMBRIGHT Family Probe)250-1500slides6766 4398
CSK-PHDG1-AActi-stain? 488 phalloidin1x500ul(300slides)3576 2324
CSK-PHDH1-AActi-stain? 555 phalloidin1x500ul(300slides)3576 2324
CSK-PHDN1-AActi-stain? 670 phalloidin1x500ul(300slides)3576 2324
CSK-PHDR1Rhodamine Phalloidin (14 ?M in methanol)1x500ul3576 2324

* 本活动最终解释权归艾美捷科技所有 


产品应用详情了解: 


1.小G蛋白活化检测:

小G蛋白(Small G Protein)因分子量只有20-30KD而得名,同时具有GTP酶活性,在细胞信号通路中发挥分子开关的功能,参与调控很多生物学过程,包括分泌活动和细胞骨架的装配等。目前发现的小G蛋白包括 Rho, Rac, Arf1 & 6, Ras, Ral 等。小G蛋白的共同特点是,当结合了GTP时即成为活化形式,这时可作用于下游分子使之活化,而当GTP水解成为GDP时(自身为GTP酶)则恢复到非活化状态。    

Small G Protein.png


Pull-down和G-LISA实验都被广泛地用于研究小G蛋白的活性。Pull-down实验又叫蛋白质体外结合实验,可用于测定活性的GTPase亚型,可作为对G-LISA实验的补充。G-LISA 实验是一种改进的ELISA,可测定3-D细胞系、原代细胞裂解液及组织裂解液中有活性的GTPase,使GTPase定量的准确性增加。  


Pull-down及G-LISA实验结果展示:

Pull-down及G-LISA实验结果展示

Lanes 4 & 5:10 ng/ ml of EGF, 2minLanes 2 & 3: Persistent serum starvationLanes 1: 20 ng of recombinant Rac1-His protein run as a western blot standard

用CN01(蓝色Activators)和LPA(红色)处理后RhoA的活化进程

分别用CN01(蓝色Activators)和LPA(红色)处理后RhoA的活化进程


2.细胞组分成像结果展示:

细胞组分成像结果展示

Hela细胞有丝分裂,SiR-Tubulin染色Tubulin蛋白(绿色)

SiR和SiR700 Actin染色活细胞的Actin

SiR和SiR700 Actin染色活细胞的Actin

鬼笔环肽染色固定细胞F-Actin

鬼笔环肽染色固定细胞F-Actin

HeLa细胞有丝分裂延时成像

HeLa细胞有丝分裂延时成像,有丝分裂是一个高度敏感的过程,显示SiR-DNA染料的低细胞毒性。

 

Cytoskeleton公司成立于1993年,专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰(PTM)、细胞骨架研究相关的系列试剂盒和产品,尤其以细胞骨架相关研究见长,既能满足于样品较少的科学研究,也可以用于小规模筛选研究和高通量大规模筛选研究。   



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